de Rooij lab publications

Previous work as a foundation of our group’s focus:

Full list of publications:

Tuan-Anh Nguyen, […], Dobner, S., Babosova, O., Becirovic, T., de Rooij, L.P.M.H., […], Kubicek, S. A non-enzymatic role of Nudix hydrolase 5 in repressing purine de novo synthesis. Science 0, eadv4257, DOI:10.1126/science.adv4257

Dobner, S., Kleissl, L., Tóth, F., Paxton, R., Yordanova, I., Brazdilova, K., Vanluyten, C., Aizenshtadt, A., Toth, H., Burton, J., Kopf, A., Belyaeva, V., Ferencevic, K., Al-Nuaimi, D.A., Rendeiro, A.F., Bock, C., Kalucka, J., Krauss, S., Osmanagic-Myers, S., Ceulemans, L.J., Stary, G., Abdel Fattah, A.R., de Rooij, L.P.M.H. (2025). Uncovering the transcriptional hallmarks of endothelial cell aging via integrated single-cell analysis. bioRxiv, https://doi.org/10.1101/2025.08.18.669055   

Declercq, M., Treps, L., Geldhof, V., Conchinha, N.V., de Rooij, L.P.M.H., Subramanian, A., Feyeux,
M., Cotinat, M., Boeckx, B., Vinckier, S., Dupont, L., Vermeulen, F., Boon, M., Proesmans, M.,
Libbrecht, L., Pirenne, J., Monbaliu, D., Jockmans, I., Dewerchin, M., Eelen, G., Roskams, T.,
Verleden, S., Lambrechts, D., Carmeliet, P., Witters, P. Single-cell RNA sequencing of cystic fibrosis
liver disease explants reveals endothelial complement activation (2024). Liver International, 44,
2382-2395, https://doi.org/10.1111/liv.15963

Petrenko, O., Köningshofer, P., Brusilovskaya, K., Hofer, B.F., Bareiner, K., Simbrunner, B., Jühling,
F., Baumert, T.F., Lupberger, J., Trauner, M., Kauschke, S.G., Pfisterer, L., Simon, E., Rendeiro, A.F.,
de Rooij, L.P.M.H., Schwabl, P., Reiberger, T. Transcriptomic signatures of progressive and
regressive liver fibrosis and portal hypertension (2024). iScience, 27(3):109301,
https://doi.org/10.1016/j.isci.2024.109301

Dobner, S., Tóth, F., de Rooij, L.P.M.H.# (2024). A high-resolution view of the heterogeneous aging
endothelium. Angiogenesis, 27, 129-145, https://doi.org/10.1007/s10456-023-09904-6

AlZaim, I.*, de Rooij, L.P.M.H.*, Sheikh, B.N*., Börgeson, E.*, Kalucka, J. The evolving functions of
the vasculature in regulating adipose tissue biology in health and obesity (2023). Nature Reviews
Endocrinology, 19, 691–707, https://doi.org/10.1038/s41574-023-00893-6

Abdel Fattah, A.F., Grebeniuk, S., de Rooij, L.P.M.H., Salmon, I., Ranga, A. Neuroepithelial organoid
patterning is mediated by a neighborhood watch mechanism (2023). Cell Reports, 42, 113334,
https://doi.org/10.1016/j.celrep.2023.113334

Sokol, L., Cuypers, A., Truong, A.C.K., Bouché, A., Brepoels, K., Souffreau, J., Rohlenova, K., Vinckier,
S., Schoonjans, L., Eelen, G., Dewerchin, M., de Rooij, L.P.M.H.# & Carmeliet, P.# Prioritization and
functional validation of target genes from single-cell transcriptomics studies (2023).
Communications Biology, 6, 648, https://doi.org/10.1038/s42003-023-05006-7

Vujovic, A.,* de Rooij, L.P.M.H.,* Chahi, A.K., Chen, H.T., Yee, B.A., Loganathan, S.K., Liu, L., Chan,
D.C.H., Tajik, A., Tsao, E., Moreira, S., Joshi, P., Xu, J., Wong, N., Balde, Z., Jahangiri, S., Zandi, S.,
Aigner, S., Dick, J.E., Minden, M.D., Schramek, D., Yeo, G.W., Hope, K.J. In vivo screening unveils
pervasive RNA-binding protein dependencies in leukemic stem cells and identifies ELAVL1 as a
therapeutic target (2023). Blood Cancer Discovery, 4, 180-207, https://doi.org/10.1158/2643-
3230.BCD-22-0086

Cuypers A, Teuwen L.A., Bridgeman VL, de Rooij L.P.M.H., Eelen G, Dewerchin M, Cantelmo A.R.,
Kalucka J, Bouché A, Vinckier S, Carton A, Manderveld A, Vermeulen P.B., Reynolds A.R., Carmeliet
P. Generation of vessel co-option lung metastases mouse models for single-cell isolation of
metastases-derived cells and endothelial cells (2022). STAR Protocols, 27, 3(4):101691,
https://doi.org/10.1016/j.xpro.2022.101691

Geldhof, V.,* de Rooij, L.P.M.H.,* Sokol, L.,* Amersfoort, J.,* De Schepper, M., Rohlenova, K., Hoste,
G., Vanderstichele, A., Delsupehe, A.M., Isnaldi, E., Dai, N., Taverna, F., Khan, S., Teuwen, L.A.,
Richard, F., Treps, L., Smeets, A., Nevelsteen, I., Weynand, B., Vinckier, S., Schoonjans, L., Desmedt,
C., Neven, P., Mazzone, M., Floris, G., Punie, K., Dewerchin, M., Eelen, G., Wildiers, H., Li, X., Luo, Y.
& Carmeliet, P. Single cell atlas identifies lipid-processing and immunomodulatory endothelial cells
in healthy and malignant breast (2022). Nature Communications, 13:5511,
https://doi.org/10.1038/s41467-022-33052-y

de Rooij, L.P.M.H.,* Becker, L.M.,* Teuwen, L.A., Boeckx, B., Jansen, S., Feys, S., Verleden, S.,
Liesenborghs, L., Stalder, A.K., Libbrecht, S., Van Buyten, T., Philips, G., Subramanian, A., Dumas,
S.J., Meta, E., Borri, M., Sokol, L., Dendooven, A., Gunst, J., Van Mol, P., Haslbauer, J.D., Rohlenova,
K., Menter, T., Boudewijns, R., Geldhof, V., Vinckier, S., Van Raemdonck, D., Jacobs, W., Ceulemans,
L.J., Weynand, B., Thienpont, B., Lammens, M., Kuehnel, M., Eelen, G., Dewerchin, M., Schoonjans,
L., Jonigk, D., Van Dorpe, J., Tzankov, A., Wauters, E., Mazzone, M., Neyts, J., Wauters, J.,
Lambrechts, D. & Carmeliet, P. The pulmonary vasculature in lethal COVID-19 and idiopathic
Laura de Rooij Uncovering the heterogeneity of endothelial cell senescence
pulmonary fibrosis at single-cell resolution (2022). Cardiovascular Research, 119, 520-535,
https://doi.org/10.1093/cvr/cvac139

Wang, F., Ding, P., Xiang, X., Ding, X., Brandt, C.B., Sjostedt, E., Zhu, J., Bolund, S., Zhang, L., de Rooij,
L.P.M.H., Luo, L., Wei, Y., Zhao, W., Lv, Z., Haskó, J., Li, R., Qin, Q., Jia, Y., Wu, W., Yuan, Y., Pu, M.,
Wang, H., Wu, A., Xie, L., Liu, L., Chen, F., Herold, J., Kalucka, J., Karlsson, M., Zhang, X., Helmig, R.B.,
Fagerberg, L., Lindskog, C., Pontén, F., Uhlen, M., Bolund, L., Jessen, N., Jiang, H., Xu, X., Yang, H.,
Carmeliet, P., Mulder, J., Chen, D., Lin, L. & Luo, Y. Endothelial cell heterogeneity and microglia
regulons revealed by a pig cell landscape at single-cell level (2022). Nature Communications, 13, 1-
18, https://doi.org/10.1038/s41467-022-31388-z

de Rooij, L.P.M.H., Becker, L.M. & Carmeliet, P. A role for the vasculature endothelium in post-acute
COVID-19? (2022) Circulation, 145, 1503-1505, https://doi.org/10.1161/circulationaha.122.059231

Becker, L.M.,* Chen, S.H.,* Rodor, J.,* de Rooij, L.P.M.H.,* Baker, A.H. & Carmeliet, P. Deciphering
endothelial heterogeneity in health and disease at single cell resolution: progress and perspectives
(2022). Cardiovascular Research, 119, 6-27, https://doi.org/10.1093/cvr/cvac018

Rodor, J., Chen, S.H., Scanlon, J.P., Monteiro, J.P., Caudrillier, A., Sweta, S., Stewart, K.R., Shmakova,
A., Dobie, R., Henderson, B.E.P., Stewart, K., Hadoke, P.W.F., Southwood, M., Upton, P.D., Morrell,
N.W., Ziwen, L., Brittan, M., Chansy, S., Handen, A., Lafyatis, R., de Rooij, L.P.M.H., Henderson, N.C.,
Spiroski, A.M., Carmeliet, P., Baker, A.H. Single cell RNA-seq profiling of murine endothelial cells in
response to pulmonary arterial hypertension (2021). Cardiovascular Research, 118, 2519-2534,
https://doi.org/10.1093/cvr/cvab296

Conchinha, N.V., Sokol, L., Teuwen, L.A., Veys, K., Dumas, S.J., Meta, E., García-Caballero, M.,
Geldhof, V., Chen, R., Treps, L., Borri, M., de Zeeuw, P., Falkenberg, K.D., Dubois, C., Parys, M., de
Rooij, L.P.M.H., Goveia, J., Rohlenova, K., Schoonjans, L., Dewerchin, M., Eelen, G., Li, X., Kalucka,
J., Carmeliet, P. (2021). Protocols for endothelial cell isolation from mouse tissues: brain, choroid,
lung, and muscle. STAR protocols, 2, 100508, https://doi.org/10.1016/j.xpro.2021.100508

Dumas, S.J., Meta, E., Conchinha, N.V., Sokol, L., Chen, R., Borri, M., Teuwen, L.A., Veys, K., García-
Caballero, M., Geldhof, V., Treps, L., de Zeeuw, P., Falkenberg, K.D., Dubois, C., Parys, M., de Rooij,
L.P.M.H., Goveia, J., Rohlenova, K., Schoonjans, L., Dewerchin, M., Eelen, G., Li, X., Kalucka, J.,
Carmeliet, P. (2021). Protocols for endothelial cell isolation from mouse tissues: kidney, spleen, and
testis. STAR protocols, 2, 100523, https://doi.org/10.1016/j.xpro.2021.100523

Teuwen, L.A., de Rooij, L.P.M.H., Cuypers, A., Rohlenova, K., Dumas, S.J., García-Caballero, M.,
Meta, E., Amersfoort, J., Taverna, F., Becker, L.M., Veiga, N., Cantelmo, A.R., Geldhof, V., Conchinha,
N., Kalucka, J., Treps, L., Conradi, L.C., Khan, S., Karakach, T.K., Soenen, S., Vinckier, S., Schoonjans,
L., Eelen, G., Van Laere, S., Dewerchin, M., Dirix, L., Mazzone, M., Luo, Y., Vermeulen, P. & Carmeliet,
P. Tumor vessel co-option probed by single-cell analysis (2021). Cell Reports, 35, 109253,
https://doi.org/10.1016/j.celrep.2021.1092

Sokol, L., Geldhof, V., García-Caballero, M., Conchinha, N.V., Dumas, S.J., Meta, E., Teuwen, L.A.,
Veys, K., Chen, R., Treps, L., Borri, M., de Zeeuw, P., Falkenberg, K.D., Dubois, C., Parys, M., de Rooij,
L.P.M.H., Goveia, J., Rohlenova, K., Schoonjans, L., Dewerchin, M., Eelen, G., Li, X., Kalucka, J.,
Carmeliet, P. (2021). Protocols for endothelial cell isolation from mouse tissues: small intestine,
colon, heart, and liver. STAR protocols, 2, 100489, https://doi.org/10.1016/j.xpro.2021.100489

Chan, D.C.H., Xu, J., Vujovic, A., Wong, N., Gordon, V., de Rooij, L.P.M.H., Moreira, S., Joyce, C.E., La
Rose, J., Sandí, M.J., Doble, B.W., Novina, C., Rottapel, R.K. & Hope, K.J. Arhgef2 regulates mitotic
spindle orientation in hematopoietic stem cells and is essential for productive hematopoiesis
(2021). Blood Advances, 5, 3120-3133, https://doi.org/10.1182/bloodadvances.2020002539

Kalucka, J.*, de Rooij, L.P.M.H.*, Goveia, J., Rohlenova, K., Dumas, S.J., Meta, E., Conchinha, N.,
Teuwen, L.A., Geldhof, V., Chen, R., Sokol., L., Treps, L., Khan, S., Taverna, F., Borri, M., de Zeeuw,
P., Dubois, C., Falkenberg, K., Parys, M., Yin, X., Du, Y., Schoonjans, L., Dewerchin, M., Eelen, G., Lin,
L., Bolund, L., Li, X., Luo, Y. & Carmeliet, P. (2020). A single cell transcriptome atlas of murine
endothelial cells. Cell, 180, 764-779, https://doi.org/10.1016/j.cell.2020.01.015

Rohlenova, K., Goveia, J., García-Caballero, M., Subramanian, A., Kalucka, J., Treps, L., Falkenberg,
K., de Rooij, L.P.M.H., Zheng, Y., Lin, L., Sokol, L., Teuwen, L.A., Geldhof, V., Taverna, F., Pircher, A.,
Conradi, L.C., Khan, S., Stegen, S., Panovska, D., De Smet, F., Staal., F.J.T., McLaughlin, R.J., Vinckier,
S., Van Bergen, T., Ectors, N., De Haes, P., Wang, J., Bolund, L., Schoonjans, L., Karakach, T.K., Yang,
H., Carmetliet, G., Liu, Y., S., Thienpont, B., Dewerchin, M., Eelen, G., Li, X., Yonglun, L., & Carmeliet,
P. (2020). Single-Cell RNA Sequencing Maps Endothelial Metabolic Plasticity in Pathological
Angiogenesis. Cell Metabolism, 31, 826-877, https://doi.org/10.1016/j.cmet.2020.03.009

Goveia, J., Treps, L., Conradi, L.C., Rohlenova, K., Taverna, F., Pircher, A., Kalucka, J., Karakach, T.,
Teuwen, L.A., Geldhof, V., de Rooij, L.P.M.H., Draoui, N., Santos, I.M., Khan, S., Olbrecht, S., Boeckx,
B., Wauters, E., Decaluwe, H., de Leyn, P., Vansteenkiste, J., Sagaert, X., Verbeke, E., Vanderstichele,
A., Busschaert, P., Vinckier, S., Vergrote, I., Mazzone, M., Eelen, G., Dewerchin, M., Schoonjans, L.,
Thienpont, B., Lambrechts, D. & Carmeliet, P. (2020). An Integrated Gene Expression Landscape
Profiling Approach to Identify Lung Tumor Endothelial Cell Heterogeneity and Angiogenic
Candidates. Cancer Cell, 37, 21-36, https://doi.org/10.1016/j.ccell.2020.03.002

Dumas, S.J., Meta, E., Borri, M., Goveia, J., Rohlenova, K., Conchinha, N., Falkenberg, K., Teuwen,
L.A., de Rooij, L.P.M.H., Kalucka, J., Chen, R., Khan, S., Taverna, F., Weisi, L., Parys, M., De Legher,
C., Vinckier, S., Karakach, T.K., Schoonjans, L., Lin, L., Bolund, L., Dewerchin, M., Eelen, G., Rabelink,
T.J., Li, X., Luo, Y. & Carmeliet, P. (2020). Single-Cell RNA Sequencing Reveals Renal Endothelium
Heterogeneity and Metabolic Adaptation to Water Deprivation. JASN, 31, 118-138,
https://doi.org/10.1681/asn.2019080832

Khan, S., Taverna, F., Rohlenova, K., Treps, L., Geldholf, V., de Rooij, L.P.M.H., Sokol, L., Pircher, A.,
Conradi, L.C., Kalucka, J., Schoonjans, L., Eelen, G., Dewerchin, M., Karakach, T., Li, X., Goveia, J. &
Carmeliet, P. (2019). EndoDB: a database of endothelial cell transcriptomics data. Nucleic Acids
Research, 47, 736-744, https://doi.org/10.1093/nar/gky997

de Rooij, L.P.M.H., Chan, D.C.H., Keyvani Chahi, A. & Hope, K.J. (2018). Post-transcriptional
regulation in hematopoiesis: RNA binding proteins take control. Biochemistry & Cell Biology, 97, 10-
20, https://doi.org/10.1139/bcb-2017-0310

# (co-)corresponding author; *shared first author